in vitro effect of ?-tocopherol, ascorbic acid and lycopene on low density lipoprotein glycation

nonenzymatic glycation of low density lipoprotein (ldl) is a reaction of glucose and other reducing sugars with apolipoprotein b100 (apo-b100) lysine residues. in diabetes, this reaction is greatly accelerated and is important in the pathogenesis of diabetic complications. the objective of this study was to investigate in vitro effects of ?-tocopherol, ascorbic acid and lycopene on ldl glycation. ldl was isolated from edta-plasma by ultracentrifugation using a single step discontinuous gradient. ldl and glucose were incubated without and with different concentrations of lycopene, ascorbic acid and ?-tocopherol. ldl glycation were estimated by sodium periodate assay. based on this study results, ?-tocopherol, ascorbic acid and lycopene decrease ldl glycation in a dose dependent manner. the electrophoretic mobility of glycated ldl decreased in presence these nutrients. these effects may be due to antioxidant properties of these nutrients and may have a role in ameliorating atherosclerotic risk of patients with diabetes mellitus.